2019 Fiscal Year Final Research Report
Specificity and molecular mechanism of synapse formation
| Project/Area Number |
16H04676
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 神経科学 / シナプス形成 / 脳神経疾患 / 脳・神経 / 遺伝子 / 蛋白質 |
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| Outline of Final Research Achievements |
We found a non-canonical pathway of pre- and postsynaptic interactions by unbiased screening of synaptic molecules interacting synapse organizers, PTPδ and neurexins, using synapse inducing system in primary neuronal cultures and protein cross-linking method. The physiological significance of this new interaction between synapse organizers was revealed by analyzing mutant mice with mutations specifically disrupting the non-canonical pathway. We also found that neurexins are essential for the survival of cerebellar granule neurons by analyzing triple knockout mice lacking neurexin 1, 2 and 3.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
シナプス形成誘導時にシナプスオーガナイザーと相互作用する分子を特異的かつ網羅的に単離するunbiased screeningにより、脳シナプス形成の分子機構の解明を推進した。シナプスオーガナイザーの変異は知的障害、自閉症、統合失調症などに密接に関連することが知られており、シナプス形成の分子機構解明は、シナプス形成の破綻が原因になることが知られている精神疾患のバイオマーカーや治療薬の開発に貢献することが期待される。
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