Physiological function of the receptor expressing intestinal tuft cells and screening of its inhibitors

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H04919
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Meiji University
• Principal Investigator: Ishimaru Yoshiro 明治大学, 農学部, 専任准教授 (10451840)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 受容体 / 刷子細胞 / 代謝 / 免疫 / 遺伝子破壊マウス / 消化管

Outline of Final Research Achievements

To elucidate the function of the orphan receptor Gprc5c, which is expressed in intestinal tuft cells, in vivo, we generated its knockout mice by new genome editing methods, TALEN and CRISPER/Cas9 and analyzed its phenotypes. KO mice have reduced body weight. Infection experiments showed that Gprc5c may not be involved in type 2 innate immune response against nematode, Nippostrongylus brasiliensis, infection.

Free Research Field

食品科学

Academic Significance and Societal Importance of the Research Achievements

近年、アレルギー性疾患やメタボリックシンドロームは解決すべき重要な社会問題になっている。小腸刷子細胞の頂端部に局在するオーファン受容体の生理機能が解明され、この受容体を阻害する食品由来因子などの化合物を見つけることができれば、アレルギー性疾患や肥満・糖尿病の予防や治療に繋げることが期待される。