Differentiation and time specific gene Identification by the mapping of transcriptional start site

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H05159
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human genetics
• Research Institution: Nagasaki University
• Principal Investigator: YOSHIURA Koh-ichiro 長崎大学, 原爆後障害医療研究所, 教授 (00304931)
• Research Collaborator: KINOSHITA Akira ; MISHIMA Hiroyuki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 転写産物 / 5'末端 / switching 法 / 虹彩 / 眼

Outline of Final Research Achievements

We isolated a transcript playing key role for developing anterior segment of eyes. The transcription starts at the middle of exon coding known protein, so it was so difficult to identify the expression due to its amount of expression and its transcription start point. The protein coded by the short transcript is proved to be in gene edited mice eye harboring tag-peptides, and to be important for iris, epithelium of cornea in their early developing stage.
The switching method using reverse transcriptase from M-MLV, mouse moloney leukemia virus, is very effective method to identify 5’-end of transcript inhibiting concatemerizatio of switching oligo RNA and maxmizing the switching efficiency.

Free Research Field

分子遺伝学

Academic Significance and Societal Importance of the Research Achievements

虹彩，角膜などの眼前方部の発生に重要な遺伝子転写産物を新規に同定することが出来た。学術的には，転写開始される場所を変えた新規遺伝子が，生体内で発生に重要な新たな機能を持つことを示した。つまり，まだまだ重要な転写産物が，身悪阻されている可能性を示している。
社会的には，角膜の発生に関わる初期段階に働くタンパク質を見つけたことで，角膜再生医療における重要タンパクの一つを発見したといえる。