2018 Fiscal Year Final Research Report
Development of the Dual fluorescent cytology with tumor-specific viruses for gastrointestinal cancer precision medicine
| Project/Area Number |
16H05416
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
香川 俊輔 岡山大学, 大学病院, 准教授 (00362971)
田澤 大 岡山大学, 大学病院, 准教授 (90415513)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 癌 / 遺伝子 / 細胞・組織 / 医療・福祉 / トランスレーショナルリサーチ |
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| Outline of Final Research Achievements |
Peritoneal dissemination is the most common form of metastasis in gastrointestinal cancer, and is associated with an extremely poor prognosis. We developed a new approach to visualize floating tumor cells using a green fluorescent protein (GFP)-expressing attenuated adenovirus in which the telomerase promoter regulates viral replication (TelomeScan, OBP-401). Moreover, we used a unique TelomeScan derivative (TelomeScan-F45, OBP-1101) that have a strict replication control function by micro-RNA together with targeting properties through CD46; however, unfortunately, the combination strategy was not able to show the usefulness.
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| Free Research Field |
消化器外科学
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| Academic Significance and Societal Importance of the Research Achievements |
テロメラーゼ特異的蛍光標識ウイルス製剤TelomeScan（OBP-401）では、EpCAMなど上皮系マーカーを指標としたCellSearchシステムでは検出不能であった、より悪性化して上皮間葉転換（EMT）を生じたがん細胞も標識することができ、鋭敏で高精度な検出感度が確認できた。TelomeScan-F45（OBP-1101）を併用するデュアル体外診断システムの確立は困難であったが、TelomeScanによる臨床的に有用なプラットフォームは構築できたと考える。
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