Development of a xeno-free induction and maintenance protocol for MSCs from PSCs

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H05447
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: IKEYA Makoto 京都大学, iPS細胞研究所, 准教授 (20442923)
• Co-Investigator(Kenkyū-buntansha): ALEV CANTAS 京都大学, iPS細胞研究所, 特定拠点助教 (30726477)
• Research Collaborator: OHTA Akira ; SAKURAI Hidetoshi ; MORIZANE Asuka ; SATO Takahiko
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 間葉系幹細胞

Outline of Final Research Achievements

Aiming to the future regenerative medicine and drug discovery research, we have tried to establish efficient xeno-free induction methods for mesenchymal stem cells from iPS cells through three different cell line lineages; neural crest cells, neural cells and mesodermal cells. In addition, in order to obtain a large amount of cells in the same lot, we have tried to develop expansion culture and cryopreservation methods for neural crest cells, neural cells and mesodermal cells. The quality of the induced mesenchymal stem cells was compared and evaluated in their ability to differentiate into bone, cartilage and fat. We succeeded to establish induction methods via neural crest cells and mesodermal cells, and partially succeeded to establish an induction method via nerve cells. The expansion culture was successful for neurons, and partially successful for neural crest cells, mesodermal cells, and mesenchymal stem cells.

Free Research Field

幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

間葉系幹細胞は既に細胞移植治療などの臨床応用に使用されている有用な細胞であるにも関わらず、その分子的な実態はあまりよく分かっていない。そもそも現在の間葉系幹細胞の定義は極めて定性的であり、多種多様な細胞が含まれるため、間葉系幹細胞の本質を捉えているとは言い難い。本申請により３種類の中間細胞のうち、少なくとも神経堤細胞と中胚葉細胞について、間葉系細胞への文化誘導に成功した。最終産物の比較についてはまだ十分ではないが、これらの細胞を分化能、および遺伝子発現などによって分類する、さらに成体由来の間葉系幹細胞とも比較することによって、間葉系幹細胞の本質を明らかにできると考えられる。