Establishment of skin microbe-host immunity analysis method based on understanding the concept of "superorganism"

2018 Fiscal Year Final Research Report

• Project/Area Number: 16H06252
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Chiba University
• Principal Investigator: Matsuoka Yuumi 千葉大学, 大学院医学研究院, 講師 (10402067)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 皮膚細菌叢 / 免疫応答 / 超個体 / 黄色ブドウ球菌 / カンジタ

Outline of Final Research Achievements

Microbiome analysis is widely performed in recent years in the intestinal tract, skin, etc., and the contribution of microbiome to the diseases is becoming clearer. When we analysis the microbe-immune response in the skin, we have to analyze not only the host immune responses and bacterial community, but also fungal and viral community. However, there was no good model for microbial immune response analysis targeting whole symbiotic microorganisms. Therefore, we aim to establish a new model for analyzing the microbe-immune response in the skin. In this study, we found that IL-17-producing cells in our S. aureus infection model were ILC3 and gdT cells. In addition, a phenotypic differences were obvious in keratinocyte-specific MyD88knockout and IL-1/36 deficient mice.We also established a new inflammatory skin model with pathogenic fungi such as C. albicans and M. furfur.

Free Research Field

皮膚細菌学、免疫学

Academic Significance and Societal Importance of the Research Achievements

病原微生物による皮膚炎発症のメカニズムについてはこれまで良い動物モデルがなく、詳細は明らかではなかった。我々が先行研究で確立した経表皮的黄色ブドウ球菌感染マウスモデルを用いて皮膚の最外層における病原微生物に対する宿主応答を検討することで、新たな病原微生物による皮膚炎発症のメカニズムを明らかにできたと考えている。