Identification of the components of heat-responsible novel nuclear body containing MALAT1, a long noncoding RNA

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06743
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: Onoguchi Rena (水谷玲菜) 東京大学, アイソトープ総合センター, 特任助教 (30780697)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: ノンコーディングRNA / 核内構造体 / 熱ストレス応答

Outline of Final Research Achievements

Previously I found that localization of MALAT1 long noncoding RNA was altered and formed novel nuclear bodies in heat shock condition. In this project, I tried to identify the MALAT1-binding proteins as components of heat-inducible MALAT1 containing nuclear bodies. I initially fixed the experimental condition of affinity purification of MALAT1 RNA: proteins complexes. I identified the 70 kinds of proteins as MALAT1-binding proteins in heat shock condition. Because MALAT1-binding proteins contain the transcription factors and the splicing factors, the heat-inducible MALAT1-containing nuclear bodies may regulate transcription or splicing in heat shock response.

Free Research Field

分子生物学