2017 Fiscal Year Final Research Report
The mechanism of chemotherapy resistance in colon cancer stem cells and epithelial mesenchymal transition colon cancer cells
| Project/Area Number |
16H06899
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | がん幹細胞 / 大腸癌 / 上皮間葉形質転換 / 抗癌剤 / 治療抵抗性 |
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| Outline of Final Research Achievements |
We developed patient-derived xenograft tumor models from clinical samples of colon cancer patients. We did in vivo chemotherapy experiments using the PDX models treated with irinotecan which is one of the standard chemotherapy drugs in clinical practice of colon cancer treatment. Cancer stem cells were sorted by FACS (fluorescence activated cell sorting) and transcriptomic analysis of the sorted cells was subsequently done. Then, we have identified CDKN3 as a new mechanism of chemotherapy resistance in colon cancer cells. CDKN3 is one of the downstream targets of transcription factor E2F4 which I previously reported as a key transcription factor in the mechanism of chemotherapy resistance.
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| Free Research Field |
消化器癌の化学療法
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