Distribution of pentosidine in dentin collagen

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06959
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: Shimizu Masato 大阪大学, 歯学部附属病院, 医員 (70380277)
• Research Collaborator: MIURA Jiro 大阪大学, 歯学部附属病院, 助教 (70437383)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 象牙質 / コラーゲン / 糖化最終産物 / ペントシジン / 蛍光 / ２型糖尿病 / 加齢

Outline of Final Research Achievements

Reducing sugar binds to proteins under biological conditions, and through rearrangement and oxidation it reacts irreversibly to form advanced glycation end products (AGEs).　As dentin collagen does not undergo extensive metabolization after eruption, it has been reported that the amount of AGEs in human dentin remains stable regardless of age. However, we have observed that the amount of AGEs differs significantly between dentin from patients of different ages or from dentin in dental caries.　Analyzing the glycation process in dentin requires the calculation of the temporal correlation between glycation stress and dentin collagen. Therefore, we used type 2 diabetes model rats to analyze the temporal correlation of AGEs through the quantitation of pentosidine. The results indicated that in contrast to normal rats, the distribution of pentosidine spreads from the predentin to the enamel side in the molars of diabetes rats, and is also deposited in the periodontal tissues.

Free Research Field

保存治療系歯学