Establishment of the model of intestinal and gastric type gastric cancer using CRISPR/Cas9 and organoid

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06999
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Hiroshima University
• Principal Investigator: Sakamoto Naoya 広島大学, 医歯薬保健学研究科(医), 助教 (20571798)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: オルガノイド / CRISPR/Cas9 / ゲノム編集

Outline of Final Research Achievements

We have established the organoids from intestinal metaplasia and fundic/pyloric gland by using the selection of culture condition and FACS, and then try to introduce several genomic abnormalities that are characteristic especially in gastric cancer (GC) using CRISPR/Cas9 system. We also generated the organoids from GC cases with intestinal and gastric mucin phenotype, respectively. It was found that GC organoids with intestinal mucin phenotype showed more significant resistance against 5-FU than GC organoids with gastric mucin phenotype.

Free Research Field

病理学