2017 Fiscal Year Final Research Report
Elucidation of cancer specific energy metabolism by MIR34a and clinical application of it.
| Project/Area Number |
16H07344
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Osaka Medical College |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-08-26 – 2018-03-31
|
| Keywords | microRNA / Warburg 効果 / MIR34a / GLUT1 / LDHA / MYC |
|---|
| Outline of Final Research Achievements |
The ectopic expression of MIR34a significantly inhibited the cancer cell growth through the induction of autophagy in gastric cancer (GC) cells. The production of lactate and the up-take of glucose were decreased in MIR34a-treated GC cells through the down-regulation of the expression levels of SLC2A1 and LDHA. Also, the expression levels of LDHA and SLC2A1 were up-regulated in clinical GC samples. Our findings implied that dysregulation of MIR34a-expression affect the acquisition of the Warburg effect in GC cell. Additionally, we revealed that RNA-helicase DDX6 promote GC development by regulation of MYC. Also, we detected new PTBP1-associated miRNAs. Our findings in this project were very important for innovative drug development which targets the Warburg effect.
|
| Free Research Field |
消化器外科、核酸創薬、分子生物学
|
