Investigation of the protein quality and quantity control system in plants using a fluorescent reporter

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07441
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: HAYASHI Shimpei 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 研究員 (40781323)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 小胞体 / ストレス / タンパク質 / 植物 / 変異体

Outline of Final Research Achievements

Endoplasmic reticulum (ER) stress responses control the protein quality and quantity. To shed light on the unknown mechanism of the control system in plants, we generated transgenic Arabidopsis plants in which fluorescence proteins are induced by ER stress response. To identify novel factors involved in the control system, we mutagenized the transgenic plants and screened mutants. We successfully isolated many unique mutants that exhibited altered fluorescence pattern. Since these mutants are important clues for understanding how plant cells managing risks involved in protein production, we are carrying out the characterization of these mutant plants and mapping of the responsible genes. These data will lead to the development of a sophisticated protein production system using plants in the future.

Free Research Field

植物分子生物学