Novel Approach of Solid-State NMR to Reveal Molecular Mechanism of Supramolecular Structural Formation of Biomolecules Through Lipid Bilayers

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K05858
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Institute for Molecular Science
• Principal Investigator: Nishimura Katsuyuki 分子科学研究所, 物質分子科学研究領域, 准教授 (00334631)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 固体NMR / アミロイドβ / オリゴマー / 脂質膜 / 構造解析

Outline of Final Research Achievements

We have explored to develop effective techniques of solid-state NMR for structural characterizations of peripheral membrane proteins and peptides which express their functions based on their supramolecular structures conformed by associations among them and interactions with lipid bilayers. On the other hand, amyloid-β has been considered to play crucial roles in the onset and developments of Alzheimer's disease through the fomation of insoluble amyloid fibrils. In this study, we have also explored structural characterization of oligomeric state amyloid-β(1-40) induced on model neuronal lipid membranes based on solid-state NMR.

Free Research Field

構造生命科学

Academic Significance and Societal Importance of the Research Achievements

アルツハイマー病は、多くの国民に直接関わる重要な疾患であるが、その発症機構の解明、および完治可能な治療薬の開発には至っていない。当該疾患の発症に深く関与することが示唆されているアミロイドβが形成する不溶性アミロイド線維形成の分子機構解明は、同疾患治療および予防の実現に必要不可欠な研究課題である。本研究は、この解明に大きく寄与することが期待され、学術的、社会的にもその意義は大きいと考えられる。