Enrichment of fungal genetic resources by developing spore isolation method and culture preservation method for ectomycorrhizal mushrooms

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K08111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: Tottori University
• Principal Investigator: NAKAGIRI Akira 鳥取大学, 農学部, 教授 (70198050)
• Research Collaborator: ENDO naoki ; SATO masanori ; MAEKAWA nitaro ; SOTOME kozue
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 菌根菌 / 培養株 / 凍結保存 / 難培養性 / 難保存性

Outline of Final Research Achievements

Development of spore isolation methods for inducing germination of basidiospores from ectomycorrhizal mushrooms, which have been known as hard-to-culture fungi, followed by improving culture methods to establish strains of various mycorrhizal fungi and also by development of long-term preservation method for such hard-to-preserve fungi, then establishing a new biological resource is the purpose of this research. We isolated basidiospores of 22 genera and 115 species of ectomycorrhizal mushrooms on MNC medium, n-MNC supplemented with butylic acid or G-MNC containing gelangum instead of agar. We also found the optimum medium is different from taxon to taxon. By employing the freezing method known as the vermiculite method, we successfully froze and stored 197 strains of ectomycorrhizal fungi.

Free Research Field

菌類多様性、菌株保存

Academic Significance and Societal Importance of the Research Achievements

菌根性きのこ類はこれまで多くの種が培養されていなかった。本研究によって担子胞子から培養株が得られるようになることで、菌の培養性状や無性世代、菌糸の特徴などこれまで未知の性状を利用して、多様な菌根性きのこ類の生態や分類に関する新たな知見が得られることが期待できる。特に、単胞子分離株が得られることから、これまで不可能だった交配による近縁種間の生物学的種の判別も可能となる。また、多種多様な菌根性きのこ類の菌株が凍結保存されて、いつでも利用可能になれば、新たな生物資源として、有用生理活性物質の探索など様々な応用研究に活用されることが期待される。