2018 Fiscal Year Final Research Report

Fundamental study on the generation of reactive oxygen species in water and their effects on biological constituents by the irradiation of cold atmospheric pressure plasma

Research Project

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Project/Area Number	16K08203
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Physical pharmacy
Research Institution	Nihon Pharmaceutical University
Principal Investigator	Anzai Kazunori 日本薬科大学, 薬学部, 教授 (70128643)
Co-Investigator(Kenkyū-buntansha)	土田和徳日本薬科大学, 薬学部, 准教授 (50406633) 高城徳子日本薬科大学, 薬学部, 講師 (80424068)
Project Period (FY)	2016-04-01 – 2019-03-31
Keywords	大気圧低温プラズマ / 活性酸素種 / ヒドロキシルラジカル / スーパーオキシドラジカル / 一重項酸素 / 過酸化水素 / 脂質過酸化 / ESR
Outline of Final Research Achievements	Because application of cold atmospheric pressure plasma (CAPP) to biological samples have taken large attentions, it is important to examine the effects of various CAPP parameters on the generation of reactive species. We investigated the generation of reactive species in water by CAPP irradiation by changing the parameters: irradiation time, sample volume, and irradiation distance between the sample surface and plasma jet tip. It was confirmed that hydroxyl radical, superoxide radical, hydrogen radical, hydrogen peroxide (H2O2), singlet oxygen, NO2- are formed by the irradiation of CAPP, and that the generation depends on the irradiation distance. It was found that H2O2 is the major species formed by long distance irradiation. Irradiation by CAPP of liposomes caused TBARS in addition to lipid radical, suggesting that the peroxidation of liposomal lipids occurred via lipid radical caused by the reactive species produced by CAPP.
Free Research Field	物理系薬学
Academic Significance and Societal Importance of the Research Achievements	近年、大気圧低温プラズマ（以下プラズマ）の医療への応用が注目を浴びており、多くの研究成果が発表されているが、その基礎となるメカニズムの研究は遅れている。今回、水へのプラズマの照射が活性酸素種を生成し、それは放射線とは違って照射距離に依存してかつ表面の反応であるという基礎的な知見が得られた。この知見を基盤として、医療へのプラズマの新たな応用を考えることができるとともに、医療以外の応用にも役立てることが出来る。