2018 Fiscal Year Final Research Report
Elucidation of the mechanism of transcriptional reactivation at the M/G1 transition by a genome-wide analysis
| Project/Area Number |
16K08248
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
柏葉 脩一郎 東京理科大学, 基礎工学部生物工学科, 助教 (40735461)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 転写制御 / Mitotic bookmarking / ゲノムワイド解析 |
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| Outline of Final Research Achievements |
In mammalian cells, transcription is globally suppressed at M phase and is reactivated at G1 phase. Bookmarking factors are involved in this mechanism, but the detail of the mechanism is still unknown. In this study, We widely analyzed the genes which are reactivated at early G1 phase, and analyzed the motifs of transcription factor binding sites at the upstream region of these genes to find new bookmarking factors. We identified a transcription factor GABPA as a new bookmarking factor which binds to chromatin through M phase and regulates transcriptional reactivation at G1 phase.
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| Free Research Field |
ゲノム生物学 分子生物学 細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、新規 Bookmarking 因子として GABPA を新たに同定した。生物の発生過程において、一つの受精卵が増殖し、また、それぞれの組織で特異的な機能を持った細胞へと分化するためには、細胞周期のM/G1移行期において転写の再活性化が規則正しく行われる必要がある。また、その不備は細胞のがん化へも繋がることから、本研究結果は、細胞の分化を理解する上で重要な発見であるとともに、新たな発がん機構の解明に繋がる可能性がある。
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