Development of a small cell lung cancer treatment method being applied the disruption of its molecular basis of the neural stem cell undifferentiated maintenance factor

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K09582
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kumamoto University
• Principal Investigator: Kanako Niimori-Kita 熊本大学, 大学院生命科学研究部(医), 助教 (30457600)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 肺小細胞癌治療法

Outline of Final Research Achievements

The purpose of this study is that SMF1, an undifferentiated regulator of neural stem cells, maintains the immaturity and poorly differentiated properties of small cell lung cancer, and is to prove that it controls the malignancy of cell carcinoma.
First, we thought that cancer growth/metastasis could be suppressed by disrupting SMF1 expression, phosphorylation, and the molecular basis of its periphery. First, we were able to perform shotgun proteomics analysis for the purpose of comprehensive identification of SMF1-binding proteins in small cell lung cancer. A protein extract of a small cell lung cancer culture (H69 cells) was immunoprecipitated with anti-SMF1 antibody beads, SMF1-binding protein was eluted, and then digested with trypsin. The SMF1 binding protein was comprehensively identified by performing shotgun proteomics analysis on this using an LC-MS mass spectrometer.

Free Research Field

蛋白質科学

Academic Significance and Societal Importance of the Research Achievements

。臨床の現場において、肺小細胞癌は、特に予後が悪いことで知られており、抗癌剤を投与しても耐性の腫瘍が出現し、脳や肝臓など多臓器に転移するという問題がある。先行研究では、SMF1を欠損させると抗癌剤耐性の肺小細胞癌も死滅することから、これまで治療が難しかった肺小細胞癌、さらには悪性度の高い癌を根絶させることのできる画期的な治療法となるので、大変意義深い研究と考える。