Pathological study for new treatment using iPS cells derived from patients with atopic dermatitis

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K10136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kurume University (2017-2019); Iwate Medical University (2016)
• Principal Investigator: NUMATA SANAE 久留米大学, その他部局等, 講師 (40599312)
• Co-Investigator(Kenkyū-buntansha): 橋本 隆 久留米大学, 皮膚細胞生物学研究所, 教授 (20129597) ; TEYE KWESI 久留米大学, 皮膚細胞生物学研究所, 助教 (30599303) ; 夏秋 洋平 久留米大学, 医学部, 講師 (40389309) ; 石井 文人 久留米大学, 医学部, 准教授 (80330827)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: アトピー性皮膚炎 / iPS細胞

Outline of Final Research Achievements

First, the quality of the iPS cells in storage was confirmed by Western blotting and chromosome analysis.
In the subsequent differentiation into keratinocytes, the conventional method was improved by slightly changing the culture conditions, and efficient differentiation can now be performed constantly.
We have completed the steps from iPS cell establishment using blood that is less invasive to patients to keratinocyte differentiation, and we have also established an efficient differentiation method. We plan to continue the experiment in the future.

Free Research Field

皮膚科

Academic Significance and Societal Importance of the Research Achievements

iPS細胞からの表皮細胞への分化方法はいくつかの報告があるものの、安定して品質の良い表皮細胞を得るスタンダード法はいまだ確立していない。
今回、特殊な設備や試薬が無くても、安定して表皮細胞が得られる方法を確立した。
この方法を用いることは、アトピー性皮膚炎だけでなく、今後の皮膚科分野の研究の発展に貢献できるものと考える。