Determination of causative drugs using ex-vivo expanded skin infiltrating T cells in drug eruptions

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K10151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Fujiyama Toshiharu 浜松医科大学, 医学部附属病院, 講師 (60402301)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 薬疹 / 原因薬剤

Outline of Final Research Achievements

It is not always easy to determine the causative drugs in drug eruption cases. We usually perform DLST, a conventional method to determine the causative drug using peripheral blood mononuclear cells. Suspicious drugs are added to the cells to measure the uptake of 3H-thymidine. However, the detection rate is not satisfactory. Therefor we tried to establish new methods to determine the causative drugs by using ex-vivo expanded skin infiltrating T cells.
We successfully determine the causative drugs in more than half of our cases of drug eruption.

Free Research Field

皮膚科学

Academic Significance and Societal Importance of the Research Achievements

再発予防の観点から、薬疹の原因薬剤の同定は非常に重要だが、既存の検査方法ではその陽性率が高くないことから、同定できないケースも多数存在する。既存の方法では同定できなくても、今回我々が開発した、皮膚浸潤T細胞を用いた手法により、同定できたケースも存在し、調べた過半数のケースで同定できたことから、有用な手法と考えられる。