The research for the effects of X-rays irradiation on muscle and muscle precursor cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K10367
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Gihu University of Medical Science
• Principal Investigator: Sakurai Tomonori 岐阜医療科学大学, 保健科学部, 教授 (90400142)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: C2C12 / 筋衛星細胞 / マイオカイン / 速筋 / 遅筋 / 細胞遊走

Outline of Final Research Achievements

In this research, we investigated the effects of X-rays irradiation on muscle and muscle precursor cells. We found that the irradiation to muscle precursor cells at G1 phase and the cells cultured on collagen type I reduced the effects of irradiation on myogenic differentiation. The migration ability of muscle precursor cells decreased in irradiation at 4 and 8 Gy. The increase in Myh1 and Myh4 mRNA expressions and decrease in Myh7 mRNA expression were detected by X-rays irradiation both after and before myogenic differentiation. It means X-rays irradiation lead to the increase in fast muscle and decrease in slow muscle because Myh4, Myh1 and Myh7 encode the fastest, faster and latest muscle fibers, respectively. X-rays irradiation decreased mRNA expressions of myokines, CCL8、CXCL1 CCL2、CCL7、FGF21.

Free Research Field

放射線生物学

Academic Significance and Societal Importance of the Research Achievements

筋肉に対する放射線影響は、筋肉が放射線に強いと考えられてきたこととから、影響が全く見られないわけではないにもかかわらず進められた来なかった。本研究では、多くの観点から、また、筋肉に関する研究の最新知見である内分泌機能の観点からも含めて筋肉に対する放射線影響を検討し、影響が現れる条件を明らかにした点で意義深い。