2018 Fiscal Year Final Research Report
Analysis of EMT in CTCs isolated by a size-based microfluidic device in breast cancer patients
| Project/Area Number |
16K10487
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Research Institute for Clinical Oncology, Saitama Cancer Center |
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Principal Investigator |
NAGAI SHIGENORI 埼玉県立がんセンター(臨床腫瘍研究所), 病院 乳腺腫瘍内科, 副部長 (20458277)
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| Co-Investigator(Renkei-kenkyūsha) |
Tozuka Katsunori
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| Research Collaborator |
SUGANUMA masami
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 末梢血循環腫瘍細胞 / 上皮・間葉転換 / 乳がん / がん転移 / サイズ分画法 |
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| Outline of Final Research Achievements |
To determine EMT-related CTCs, we used a size-based microfluidic device for early and metastatic breast cancer patients. We found that the size-based device is more effective to detect CTCs than CellSearch System, and can determine not only epithelial-type CTCs, but also mesenchymal-type CTCs and SET-positive CTCs, using antibodies-cocktails. We also succeeded to analyze mRNA expression in CTCs fraction using digital PCR. Our established CTC-detection method will provide new insights for treatment of breast cancer.
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| Free Research Field |
乳癌 臨床腫瘍学 腫瘍免疫学
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| Academic Significance and Societal Importance of the Research Achievements |
本手法の開発によって、上皮性CTCだけでなく、間葉系CTC及び、SET陽性CTCなど多様なCTC亜集団を高い感度と特異性で検出できること可能となった。SET陽性CTCの検出は、SET阻害剤を用いた新しい乳がん治療の可能性も示唆している。本研究成果により、乳がん患者の予後予測だけでなく、がん治療の効果予測、さらには、がん転移の機構解明に役立つと期待される。
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