The elucidation of biliary and pancreatic tumor's pathobiology through the functional analysis of novel Warburg effect-associated gene PTBP1

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K10615
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Osaka Medical College
• Principal Investigator: Asakuma Mitsuhiro 大阪医科大学, 医学部, 講師 (40559390)
• Co-Investigator(Kenkyū-buntansha): 赤尾 幸博 岐阜大学, 大学院連合創薬医療情報研究科, 特任教授 (00222505) ; 廣川 文鋭 大阪医科大学, 医学部, 准教授 (20322373) ; 内山 和久 大阪医科大学, 医学部, 教授 (80232867)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 膵癌 / PTBP1 / ワールブルク効果 / スプライサー

Outline of Final Research Achievements

Previously, we indicated that the expression of PTBP1 was increased in colorectal tumor and gastric cancer. In this project, the expression of PTBP1 was increased in pancreatic cancer. Our findings suggest that PTBP1 is universal oncogene in various cancer. Knockdown of PTBP1 induced growth suppression in pancreatic cancer cells and this result suggest that PTBP1 has cancer promoted function. We performed mRNA sequence analysis in PTBP1-silencing pancreatic cancer cells and detected the PTBP1 associated genes. Also, the expression profiles of PTBP1 in normal tissues or fatal reaction of PTBP1 were revealed through the bioinformatics analysis.

Free Research Field

Oncology (pancreas)

Academic Significance and Societal Importance of the Research Achievements

PTBP1は普遍的がん遺伝子の可能性が示唆され、様々ながん種における病態解析や新規治療標的、バイオマーカーとして新規標的遺伝子となりうることが明らかとなった。また、PTBP1はがんエネルギー代謝に深く関連する遺伝子であり、PTBP1の機能解析を通じて、膵癌の病態が新たな観点か明らかになったと考えられる。