The kinetics and role of immune cells in the ischemic stroke rats model

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K10721
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Osaka University
• Principal Investigator: Sugimoto Kana 大阪大学, 医学系研究科, 講師 (00581034)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 脳梗塞 / 磁気共鳴画像法 / 免疫細胞動態 / マクロファージ

Outline of Final Research Achievements

In the present study, we confirmed the transmigration of immune cells in the ischemic stroke mice model, but did not it in the ischemic stroke rats model using 11.7T MRI imaging. Next, we tried to evaluate the role of resident microglia-derived macrophage-like cells (MG-MΦ) and bone marrow-derived macrophages (BM-MΦ) in the ischemic brain. We found that MG-MΦ signature genes were highly expressed in the ischemic core in aggravated rats, while BM-MΦ signature genes were weakly expressed. These findings suggest that the distribution ratio between two macrophages may affect progress after stroke.

Free Research Field

脳神経科学

Academic Significance and Societal Importance of the Research Achievements

本研究から、脳梗塞巣に集積する常在性マイクログリア由来マクロファージ（MG-MΦ）及び骨髄由来の浸潤性マクロファージ（BM-MΦ）の構成比率が脳梗塞の予後を大きく左右することを明らかにした。従って、今後は脳梗塞の予後を悪化させるMG-MΦの構成比率の低下、または、MG-MΦの機能抑制をターゲットとした治療法の開発を行うことが、脳梗塞の新規治療薬の開発につながると考えている。