Verification of the bioactive effect of human amnion-derived extracellular matrix-coated PLGA scaffold and its application for cartilage repair therapy.

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K10895
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Toyama
• Principal Investigator: NOGAMI Makiko 富山大学, 附属病院, 助教 (30750202)
• Research Collaborator: KIMURA tomoatsu ; SEKI shoji ; YAHARA yasuhito ; IWASAKI mami
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 軟骨再生 / 羊膜 / 生体材料 / 細胞外マトリックス

Outline of Final Research Achievements

We develop a novel cell-free biological scaffold “ECM-PLGA” for cartilage repair therapy. ECM-PLGA contains amnion-derived extra-cellular matrix-coated on bioabsorbable PLGA scaffold. Amniotic membrane has been thought to have several biological activities such as wound healing and anti-inflammation effect. This study was carried out to verify the hypothesis, anti-inflammation effect brought by amniotic ECM support the better cartilage repair in chronic osteoarthritis. When cultured on amnion-ECM coated dishes and ECM-PLGA, human synovial fibroblasts and human macrophages showed higher production of inflammatory cytokines. Gene expression pattern showed no evidence of conversion from inflammatory macrophages to anti-inflammatory macrophages. In conclusion, no obvious anti-inflammatory effect was indicated from this study.

Free Research Field

軟骨再生医学、関節病学、膝関節外科

Academic Significance and Societal Importance of the Research Achievements

羊膜は炎症抑制・創傷治癒効果に優れるとされ、再生医療分野への応用が期待される。我々が作成したECM-PLGAは過去の動物実験で優れた軟骨修復促進効果を示したが、そのメカニズムは不明である。今回in vitro実験系でECM-PLGAが軟骨欠損に伴う関節内炎症環境における抗炎症・軟骨修復に寄与する結果を期待したが、ECM-PLGAを配した培養上清中の炎症性サイトカイン分泌が高い結果となった。引き続きECM-PLGAの生体活性に関して調査を進め軟骨修復に関わるメカニズムを明らかにし、軟骨再生治療における新たな生体移植材料としてECM-PLGAの臨床応用を目指す一助としたい。