Development of the innovative treatment based on the search of microRNA blocking growth signals in urothelial carcinoma

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K11015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kagoshima University
• Principal Investigator: YOSHINO Hirofumi 鹿児島大学, 医歯学域附属病院, 助教 (90642611)
• Co-Investigator(Kenkyū-buntansha): 関 直彦 千葉大学, 大学院医学研究院, 准教授 (50345013)
• Research Collaborator: Matsushita Ryousuke ; Miyamoto Kazutaka ; Yonemori Masaya ; Enokida Hideki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: マイクロRNA / 尿路上皮癌 / miRNA-199 / miRNA-223

Outline of Final Research Achievements

We elucidated that expression levels of miRNA-199a-3p/-5p, miRNA-199b-3p/-5p, and miRNA-223 were significantly down regulated in bladder cancer tissues, and that transduction of these miRNA suppressed cell proliferation, migration, and invasion. We also elucidated that miRNA-199 and miRNA-223 individually controlled ITGA3 and WDR62 directly. We finally succeeded to publish these data in journals.
In addition, we succeeded in the establishment of cancer cells which resistant to gemcitabine or cisplatin, and made profiles of miRNA with performed next-generation sequencing analyses. We found that miRNA-486-5p was down regulated in cisplatin-resistant cancer cells according to the profiles, and that miRNA-486-5p transduction suppressed cell proliferation in these cells.

Free Research Field

urological cancer

Academic Significance and Societal Importance of the Research Achievements

1「治療標的探索型miRNA」を起点として、尿路上皮癌の再発や治療抵抗性に関わる機能性RNA分子ネットワークの探索を行い、いくつかの治療標的型miRNAを同定し論文化することができた。これらの成果により、miRNAを用いた尿路上皮癌の治療の可能性が示唆された。