The study of human trophoblast differentiation

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K11118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Fujita Health University
• Principal Investigator: Ishihara Naoe (琴村直恵) 藤田医科大学, 医学部, 研究員 (50571791)
• Co-Investigator(Kenkyū-buntansha): 原田 信広 藤田医科大学, 医学部, 教授 (00189705) ; 石原 悟 藤田医科大学, 医学部, 講師 (00300723)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 胎盤 / 栄養膜細胞 / 分化 / アロマターゼ

Outline of Final Research Achievements

Human choriocarcinoma JEG-3 cells have been used as a model system for researching differentiation of trophoblast cells. When JEG-3 cells were cultured in a differentiation condition, the cells were not uniformly differentiated; some of them showed condensed nuclei and/or aromatase expression, which are known as differentiation features of trophoblast cells. In this study, to separate differentiated and undifferentiated cells, we established cell lines transfected with fluorescent reporters, which allowed us to observe nuclear shape and aromatase expression.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

レポーター細胞株を用いて未分化及び分化細胞を分取できれば、分化関連因子を同定出来る可能性がある。それにより、胎盤の主要構成細胞である栄養膜細胞の分化過程を解明に寄与することが出来ると考える。