2018 Fiscal Year Final Research Report

Roles Gli1-positive dental pulp stem cells during dentin regeneration

Research Project

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Project/Area Number	16K11664
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Dental engineering/Regenerative dentistry
Research Institution	Health Sciences University of Hokkaido (2017-2018) Matsumoto Dental University (2016)
Principal Investigator	Hosoya Akihiro 北海道医療大学, 歯学部, 准教授 (70350824)
Co-Investigator(Kenkyū-buntansha)	吉羽邦彦新潟大学, 医歯学系, 教授 (30220718) 中村浩彰松本歯科大学, 歯学部, 教授 (50227930) 平賀徹松本歯科大学, 歯学部, 教授 (70322170) 溝口利英東京歯科大学, 歯学部, 講師 (90329475) 建部廣明北海道医療大学, 歯学部, 助教 (40638293) 入江一元北海道医療大学, 歯学部, 教授 (70223352)
Project Period (FY)	2016-04-01 – 2019-03-31
Keywords	Gli1
Outline of Final Research Achievements	Although pluripotent stem cells exist in dental pulp, the characteristics of these cells and the mechanisms of their differentiation into odontoblasts are controversial. In this study, we found that cells expressing the transcription factor Gli1 localized around the blood vessels in the dental pulp of mature mouse molar. In addition, these cells had high CFU-F activity as well as multipotency into osteoblasts, chondrocytes, and adipocytes in vitro. We also showed that Gli1-positive cells proliferate and differentiate into reparative odontoblasts during dentin regeneration after cavity formation. Therefore, these results suggest that Gli1 is a useful marker for dental pulp stem cells in the research of pulp biology.
Free Research Field	口腔解剖学
Academic Significance and Societal Importance of the Research Achievements	歯の内部にある歯髄という組織に，幹細胞が存在することが知られている。しかしこれまで，どの細胞が幹細胞であるかを指し示すことは出来なかった。本研究では，Gli1という転写因子を発現する歯髄細胞が幹細胞の性質を有し，歯髄損傷後に増殖，分化して，象牙質を再生することが示された。今後，Gli1を指標として歯髄幹細胞の研究が進展することが期待される。