Induction of iPS cell-derived lung epithelial cells by using the basement membrane substratum

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12613
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental impact assessment
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: Ito Tomohiro 国立研究開発法人国立環境研究所, 環境リスク・健康研究センター, 主任研究員 (60391067)
• Research Collaborator: MOCHIDATE Katsumi ; SONE Hideko
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 基底膜基質 / 肺上皮細胞 / iPS細胞

Outline of Final Research Achievements

High efficient induction of target cells from iPS cells is important for utilizing iPS cells in the drug discovery and toxicological analysis fields. Generally, differentiation of iPS cells are performed by culture on the components of basement membrane, such as Matrigel. On the other hand, we used the synthesized basement membrane substratum (sBM) formed by alveolar epithelial cells in the presence of Laminin expressing cells, and studied the effectiveness of sBM on the differentiation. Our data suggest that use of Laminin511-based sBM improves the differentiation of iPS cells toward lung epithelial cells at the later stage.

Free Research Field

毒性学