Expression of prenyltransferase gene in rubber degrading actinomycete

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K12631
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental conscious materials and recycle
• Research Institution: Nagaoka University of Technology
• Principal Investigator: Kasai Daisuke 長岡技術科学大学, 工学研究科, 准教授 (80452085)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: ゴム分解細菌 / プレニルトランスフェラーゼ

Outline of Final Research Achievements

Natural rubber from H. brasiliensis is used industrially for tires, seismic isolation rubbers, and medical gloves. The waste of these products have been treated by combustion or landfill processes, which are hazardous to the environment. It is required to find alternative treatment process for rubber waste. In the present study, the characterization of the rubber degradation mechanism of natural rubber degrading actinomycete and the expression of prenyltransferase which has the activity to polymerize isoprene unit, in the rubber degrader were carried out, in order to establish alternative recycle system for rubber waste. When the prenyltransferase gene from Gordonia which was cloned to pNC9503 was introduced into the cells of the rubber degrader, the expression of the gene was found. Further improvement of the gene expression will be needed to develop the recycle system for rubber waste using the rubber degrading actinomycete.

Free Research Field

応用微生物学