Development of a microchip for capture and disruption of extracellular vesicles toward cancer point-of-care diagnosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12901
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo University of Science
• Principal Investigator: Ishihara Ryo 東京理科大学, 基礎工学部材料工学科, 助教 (30633507)
• Co-Investigator(Kenkyū-buntansha): 中島 忠章 東京理科大学, 基礎工学部生物工学科, 助教 (40631213)
• Co-Investigator(Renkei-kenkyūsha): KIKUCHI Akihiko 東京理科大学, 基礎工学部 材料工学科, 教授 (40266820) ; TOMOOKA Yasuhiro 東京理科大学, 基礎工学部 生物工学科, 教授 (10197949) ; MAEDA Mizuo 理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657) ; HOSOKAWA Kazuo 理化学研究所, 前田バイオ工学研究室, 専任研究員 (00373366)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 表面機能化自律駆動マイクロチップ / 放射線グラフト重合法 / 捕捉 / その場診断 / がん

Outline of Final Research Achievements

Extracellular vesicles (EVs), which are secreted from cells to body fluids, are expected as new biomarkers because they have information of the parent cells. In this study, to use the information of the EVs for a cancer point-of-care diagnosis, we have been developed microchips for capture and disruption of the EVs. We immobilized antibody, which specifically binds to membrane protein on EVs, to inner surfaces of microchannels of a microchip modified by radiation-induced graft polymerization. Thus, a surface-functionalized power-free (SF-PF) microchip for EV capture was obtained. On the SF-PF microchip, easy and rapid EV capture was demonstrated. These results were published in journals and newspapers. In addition, we also received conference awards. Furthermore, EV disruption on a polymer chain grafted microchannel inner surfaces was also demonstrated. Improvements and integration of the above technologies would contribute to the establishment of a cancer point-of-care diagnosis.

Free Research Field

機能性高分子材料