2017 Fiscal Year Final Research Report
A stusy on biological function of endogenous CO as a byproduct of heme metabolism
| Project/Area Number |
16K13092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 一酸化炭素 / ヘム / シクロデキストリン / ポルフィリン / モデル錯体 |
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| Outline of Final Research Achievements |
Carbon monoxide (CO) is continuously produced in mammalian cells during heme metabolic reaction. In this study, we investigated the biological function of the endogenous CO using a selective CO removal agent hemoCD. In order to introduce hemoCD into the cells, we conjugated hemoCD with octaarginine peptide. The new hemoCD derivative (R8-hemoCD) was taken by living cells and captured CO in cells, in which reactive oxygen species level was significantly enhanced in the CO-removed cells. In addition, we newly synthesized a water-soluble biomimetic model for cytochrome c oxidase to investigate the reactivity of CO with a Fe/Cu hetero-binuclear structure of CcO.
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| Free Research Field |
生体関連化学
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