2017 Fiscal Year Final Research Report
Development of novel methods for efficient derivery system of functional molecular probes in plant cells
| Project/Area Number |
16K13093
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Chemical biology
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | ケミカルバイオロジー / ライブセルイメージング / 細胞内分子送達 |
|---|
| Outline of Final Research Achievements |
In plant biology, calcium ions are involved in a variety of intriguing biological phenomena as a secondary messenger. However, most of conventional calcium indicators are not applicable for plant cells partially due to the cell wall and vacuole as the specific organelle of plant cells. Therefore, for broad application with various plants in which genetic transformation is presently infeasible, useful synthetic indicators that localize in the cytosol are highly desirable.
In this research, we developed a ligand-tethered calcium indicator suitable for applications in plant cell biology by using a natural product-based ligand of cytosolic protein. This probe showed efficient cell permeability and localized within the cytosol and nucleus by means of ligand-protein interaction, and thus enabled spatiotemporal monitoring of calcium ions in the model plant cells.
|
| Free Research Field |
ケミカルバイオロジー
|
