Development of two-dimensional DNA origami structure for construction of molecular delivery system

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14033
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kyoto University
• Principal Investigator: Endo Masayuki 京都大学, 理学研究科, 特定准教授 (70335389)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: DNAナノテクノロジー / DNAオリガミ / リポソーム / 脂質膜 / 原子間力顕微鏡 / デリバリーシステム / 細胞機能制御

Outline of Final Research Achievements

In this study, we aimed to develop new strategy by constructing DNA nanostructures which can penetrate the cell membrane and application to molecular delivery system. We designed and constructed a DNA origami structure having a needle-like portion that can penetrate the lipid membrane, and observed that it interacts with the lipid bilayer membrane and forms a lattice assembly by high-speed AFM. By using liposome which is a model system of the cell, the target molecule was successfully introduced into the liposome and biochemical reaction was initiated by penetrating the lipid membrane. Using this system, we develop a new system that can introduce the target molecule from the cell surface into the cell.

Free Research Field

生物有機化学