Devising a novel light-regulated channel based on minimum unit of voltage sensor domain

2018 Fiscal Year Annual Research Report

• Project/Area Number: 16K14555
• Research Institution: Osaka University
• Principal Investigator: 岡村 康司 大阪大学, 医学系研究科, 教授 (80201987)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 電位依存性プロトンチャネル / 精子特異的カチオンチャネル

Outline of Annual Research Achievements

電位依存性プロトンチャネルHv1のチャネル機能をもつ最小ユニット分子として、S1-S2の細胞外領域を大きく欠失させ、S4の下流にある程度の細胞質側に数アミノ酸残した分子を同定することに成功した。今後これをもとにイオン透過性の変更とLOVドメインとの融合による光感受性の解析を行う予定である。精子由来カチオンチャネルのCatSperの電位センサードメインについてはホヤ由来の分子がこれまでしられているCa透過性チャネルのうち分子量最小の分子であることを示してきたが、マウス由来オルソログについても同様にCa透過性を示すことが明らかになり、今後これらの比較によりCa透過性に関わるアミノ酸部位の特定が可能になる。

Research Products

• [Journal Article] Molecular mechanisms of coupling to voltage sensors in voltage-evoked cellular signals (2019)
  • Author(s): OKAMURA Yasushi、OKOCHI Yoshifumi
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 95 Pages: 111～135
  • DOI: 10.2183/pjab.95.010
  • Peer Reviewed
• [Journal Article] The hydrophobic nature of a novel membrane interface regulates the enzyme activity of a voltage-sensing phosphatase (2018)
  • Author(s): Kawanabe Akira、Hashimoto Masaki、Nishizawa Manami、Nishizawa Kazuhisa、Narita Hirotaka、Yonezawa Tomoko、Jinno Yuka、Sakata Souhei、Nakagawa Atsushi、Okamura Yasushi
  • Journal Title: eLife Volume: 7 Pages: e41653
  • DOI: 10.7554/eLife.41653
  • Peer Reviewed / Open Access
• [Journal Article] Zn2+-Binding to the Voltage-Gated Proton Channel Hv1/VSOP (2018)
  • Author(s): Iwaki Masayo、Takeshita Kohei、Kondo Hiroko X.、Kinoshita Kengo、Okamura Yasushi、Takano Yu、Nakagawa Atsushi、Kandori Hideki
  • Journal Title: The Journal of Physical Chemistry B Volume: 122 Pages: 9076～9080
  • DOI: 10.1021/acs.jpcb.8b04890
  • Peer Reviewed
• [Journal Article] Tracking the sarcoplasmic reticulum membrane voltage in muscle with a FRET biosensor (2018)
  • Author(s): Sanchez Colline、Berthier Christine、Allard Bruno、Perrot Jimmy、Bouvard Clement、Tsutsui Hidekazu、Okamura Yasushi、Jacquemond Vincent
  • Journal Title: The Journal of General Physiology Volume: 150 Pages: 1163～1177
  • DOI: 10.1085/jgp.201812035
  • Peer Reviewed
• [Journal Article] Conservation of the Ca2+-permeability through the voltage sensor domain of mammalian CatSper subunit (2018)
  • Author(s): Arima Hiroki、Tsutsui Hidekazu、Okamura Yasushi
  • Journal Title: Channels Volume: 12 Pages: 240～248
  • DOI: 10.1080/19336950.2018.1476791
  • Peer Reviewed / Open Access
• [Journal Article] Induction of divalent cation permeability by heterologous expression of a voltage sensor domain (2018)
  • Author(s): Arima Hiroki、Tsutsui Hidekazu、Sakamoto Ayako、Yoshida Manabu、Okamura Yasushi
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Biomembranes Volume: 1860 Pages: 981～990
  • DOI: 10.1016/j.bbamem.2018.01.004
  • Peer Reviewed
• [Journal Article] Dynamic structural rearrangements and functional regulation of voltage-sensing phosphatase (2018)
  • Author(s): Sakata Souhei、Okamura Yasushi
  • Journal Title: The Journal of Physiology Volume: 597 Pages: 29～40
  • DOI: 10.1113/JP274113
  • Peer Reviewed
• [Journal Article] Voltage-Sensing Phosphatases: Biophysics, Physiology, and Molecular Engineering (2018)
  • Author(s): Okamura Yasushi、Kawanabe Akira、Kawai Takafumi
  • Journal Title: Physiological Reviews Volume: 98 Pages: 2097～2131
  • DOI: 10.1152/physrev.00056.2017
  • Peer Reviewed
• [Journal Article] 脂質－イオンチャネル相互連関 (2018)
  • Author(s): 岡村康司、大澤匡範
  • Journal Title: 「脂質クオリティ」実験医学増刊 Volume: vol.36, No.10 Pages: 97-104
  • DOI: ISBN:978-4-7581-0371-8
  • Peer Reviewed
• [Presentation] Zn2+ sensitivity of Hv1 channel: an evolutionary perspective (2019)
  • Author(s): Adisorn Ratanayotha, Takafumi Kawai, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] Cytoplasmic conformational changes of VSP detected by voltage clamp fluorescence spectroscopy (2019)
  • Author(s): Akira Kawanabe, Tomoko Yonezawa, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] The comparison of sensitivity between NaPi-IIa and NaPi-IIb activity to phosphoinositides (2019)
  • Author(s): Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] Hv1/VSOP voltage-gated proton channel inhibits migration in response to fMLF in neutrophils (2019)
  • Author(s): Yoshifumi Okochi, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] A new mechanism of Ca2+-independent voltage-dependent secretion in dorsal root ganglion neurons (2019)
  • Author(s): Zhuan Zhou, Yuan Wang, Hiroki Arima, Rong Huang, Yuqi Hang, Xingyu Du, Feipeng Zhu, Zuying Chai, Changhe Wang, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] Functional analysis of voltage-sensing phosphatase in mouse sperm (2019)
  • Author(s): Takafumi Kawai, Haruhiko Miyata, Hiroki Nakanishi, Souhei Sakata, Yoshifumi Okochi, Masahiko Watanabe, Kenji Sakimura, Takehiko Sasaki, Masahito Ikawa, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] Electrophysiological characterization of two types of Na-Pi cotransporters (2018)
  • Author(s): Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Regulation of two-step cytoplasmic conformational changes of voltage sensing phosphatase (2018)
  • Author(s): Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Toward understanding of membrane potential in phagosomai membrane (2018)
  • Author(s): Yoshifumi Okochi, Hidekazu Tsutsui, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Rapid gating of sea squirt Nav1 channel does not require auxiliary subunit (2018)
  • Author(s): Takafumi Kawai, Natsuki Eguchi, Masaki Hashimoto, Yuki Yabuuchi, Yuka Jinno, Akira Kawanabe, Junko Nishino, Atsuo Nishino, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Metal binding to the voltage-gated proton channel Hv1/VSOP (2018)
  • Author(s): Masayo Iwaki, Kohei Takeshita, Hiroko X. Kondo, Kengo Kinoshita, Yasushi Okamura, Yu Takano, Atsushi Nakagawa, Hideki Kandori
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] 電位依存性プロトンチャネルHv1/VSOPは、活性酸素の産生量の制御を介し て、好中球の走化性を抑制する (2018)
  • Author(s): 大河内善史、岡村康司
  • Organizer: 第111回 近畿生理学談話会
• [Presentation] 電位依存性プロトンチャネルは肝臓の糖新生を調節する (2018)
  • Author(s): 香山建斗、河合喬文、辰巳翔基、山本浩靖、崎村建司、木原進士、岡村康司
  • Organizer: 第111回 近畿生理学談話会
• [Presentation] Na-Pi cotransporter活性のPI(4,5)P2に対する感受性の解析 (2018)
  • Author(s): 水谷夏希、大河内善史、岡村康司
  • Organizer: 第111回 近畿生理学談話会
• [Presentation] 脊椎動物の稠密な脳の起源をホヤのNav チャネルから理解する (2018)
  • Author(s): 江口 夏生、神野 有香、橋本 真宜、河合 喬文、西野 純子、大塚 幸雄、川鍋 陽、西野 敦雄、笹倉 靖徳、岡村 康司
  • Organizer: 第4回ホヤ研究会
• [Presentation] 電位依存性ホスファターゼVSPの疎水性膜界面の役割 (2018)
  • Author(s): 川鍋 陽, 橋本 真宜, 米澤 智子, 神野 有香, 岡村 康司
  • Organizer: 第91回日本生化学会大会
• [Presentation] The analysis of sensitivity of Na-Pi cotransporter activity to phosphoinositides (2018)
  • Author(s): Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
  • Organizer: Europhysiology 2018
  • Int'l Joint Research
• [Presentation] Age-dependent regulatory function of microglial voltage-gated proton channels (2018)
  • Author(s): Takafumi Kawai, Keizo Takao, Kenji Sakimura, Tsuyoshi Miyakawa, Yasushi Okamura
  • Organizer: 第41回日本神経科学大会
• [Presentation] Sea squirt voltage-gated sodium channel : kinship with the origin of vertebrate neural sodium channels (2018)
  • Author(s): Natsuki Eguchi, Masaki Hashimoto, Yuka Jinno, Akira Kawanabe, Takafumi Kawai, Yasushi Okamura
  • Organizer: 第41回日本神経科学大会
• [Presentation] Mechanisms and physiological roles of voltage-sensing phosphatase (2018)
  • Author(s): Yasushi Okamura, Akira Kawanabe, Kazuhisa Nishizawa, Takafumi Kawai
  • Organizer: The 49th NIPS International Symposium
  • Invited
• [Presentation] 電位依存性ホスファターゼVSP：14年を経て見えたこと、見えないこと (2018)
  • Author(s): 岡村 康司、河合 喬文、川鍋 陽、筒井 秀和、坂田 宗平、佐々木 雄彦
  • Organizer: 心血管膜輸送研究会2018「心臓・血管系の頑健性と精緻な制御を支える分子基盤の統合的解明」
  • Invited
• [Presentation] 電位依存性ホスファターゼにおける膜電位変化から脱リン酸化反応の変換機構 (2018)
  • Author(s): 岡村 康司, 川鍋 陽, 坂田 宗平, 神野 有香
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] Coupling from electric signal to lipid signal; voltage-sensing phosphoinositide phosphatase (2018)
  • Author(s): Yasushi Okamura
  • Organizer: Institut NeuroMyoG?ne, LES S?MINAIRES DE L’INMG
  • Invited
• [Presentation] Ion-permeable voltage sensor domains (2018)
  • Author(s): Yasushi Okamura
  • Organizer: WCP2018 KYOTO Satellite Symposia
  • Invited
• [Presentation] 膜電位感受性イノシトールリン脂質ホスファターゼの動作原理とその応用 (2018)
  • Author(s): 岡村 康司
  • Organizer: 2018年 生理学研究所研究会 オルガネラ膜ナノドメインの機能と動態
  • Invited
• [Book] ガイトン生理学 原著第13版 (2018)
  • Author(s): 石川義弘、岡村康司（総監訳）、尾仲達史、河野憲二
  • Total Pages: 1100
  • Publisher: エルゼビア・ジャパン株式会社
  • ISBN: 9784860347741