Optogenetic manipulation of protein palmitoylation for synapse organization

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14560
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: Masaki Fukata 生理学研究所, 分子細胞生理研究領域, 教授 (00335027)
• Research Collaborator: FUKATA Yuko 生理学研究所, 分子細胞生理研究領域, 准教授 (40416186) ; YOKOI Norihiko 生理学研究所, 分子細胞生理研究領域, 助教 (50710969) ; HIRATA Tetsuya 生理学研究所, 分子細胞生理研究領域, 特任助教 (90780651) ; INAHASHI Hiroki ; KANADOME Takashi ; SUZUKI Yumi ; WATANABE Mie
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: タンパク質 / 光科学 / LOV2 / ABHD17 / 酵素 / シナプス / LOVTRAP / Zdk1-iLID

Outline of Final Research Achievements

The neuronal synapse comprises specialized membrane domains and their dynamic remodeling plays an important role in synaptic plasticity. So far, we proposed that the dynamic change in (de)palmitoylating enzyme activity at local synapses is a critical factor for reorganization of synaptic membrane domains. In this study, we aimed at creating the optogenetic probes that spatio-temporally manipulate the (de)palmitoylating enzyme activity. Taking advantage of LOV2, CRY2-CIB1, LOVTRAP and Zdk1-iLID systems, we got a promising clue.

Free Research Field

神経科学、細胞生物学、生化学