Analysis of tumor heterogeneity in peritoneal dissemination of scirrhous gastric carcinoma by multi-color fluorescent imaging

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14622
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Sasaki Foundation (2017); National Cancer Center Japan (2016)
• Principal Investigator: Yamaguchi Hideki 公益財団法人佐々木研究所, 附属研究所, 部長(移行) (10345035)
• Research Collaborator: SAITO Emi ; OKABE Akira ; KOSAKAI Ruri
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: スキルス胃癌 / 腹膜播種 / 蛍光イメージング / 腫瘍不均一性

Outline of Final Research Achievements

Scirrhous gastric carcinoma (SGC) rapidly and diffusively infiltrates in the gastric submucosa and frequently causes peritoneal dissemination. To understand the process of SGC peritoneal dissemination, we investigated heterogeneity/clonality of peritoneal tumors by multicolor fluorescent imaging. SGC cells were clonally and fluorescently marked by RGB marking method for cell lineage tracking. RGB-marked cells were i.p. or orthotopically injected into nude mice and peritoneal tumors were analyzed by fluorescent microscopy. As a result, each of the peritoneal tumors displayed multiple colors, demonstrating that they were comprised of multiclonal and heterogenous cell populations.

Free Research Field

腫瘍生物学