2017 Fiscal Year Final Research Report
gene introduction using myeloid lineage cells
| Project/Area Number |
16K14634
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor therapeutics
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Research Collaborator |
Tashiro Haruko 帝京大学, 医学部, 講師
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | CD34 / CD47 / 造血幹細胞 / 遺伝子導入 |
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| Outline of Final Research Achievements |
We constructed shuttle vector that expressed antisense nad miRNA RNA for human CD47. This vector was introduced into CD13 and CD33-positive bone marrow mononuclear cell fractions which was isolated with magnetic beads. The positive cell-fraction was cultured with recombinant human granulocyte colony-stimulating factor. The obtained mature neutrophilic granulocytes expressed anti CD47 antisense RNA and miRNA. Biological antitumor effects were also elevated.
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| Free Research Field |
造血幹細胞
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