2016 Fiscal Year Final Research Report
Development of a rapid diagnostic for detecting single base mutation related to viral drug-resistance
| Project/Area Number |
16K14651
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | Osaka University |
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Principal Investigator |
Kaihatsu Kunihiro 大阪大学, 産業科学研究所, 特任准教授(常勤) (70419464)
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| Research Collaborator |
TAKENAKA Aya
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Keywords | 遺伝子 / 核酸 / 一塩基多型 / 薬剤耐性 |
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| Outline of Final Research Achievements |
Current virus detection from clinical sample relies on immunochromatography that captures and detects its target viral protein by gold nanoparticle-conjugate antibody. On the other hand, we have established a method to capture the target viral gene within the viral particle by short oligonucleotide and visualized the gene using gold nanoparticles.
In this study, we succeeded to distinguish a drug-resistance of influenza virus by improving the sequence specificity of short oligonucleotide with a simple chemical modification. We further visually distinguished drug-resistant and drug-sensitive influenza virus on a membrane by our chemically-modified nucleic acid.
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| Free Research Field |
核酸化学
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