2016 Fiscal Year Final Research Report
Verification of genomic diversity required for tumorigenesis
| Project/Area Number |
16K14664
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Keywords | ゲノム編集 / CRISPR/Cas9システム / 超並列シークエンス解析 / 腫瘍形成能 |
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| Outline of Final Research Achievements |
CRISPR/Cas9 library was introduced to NIH3T3 cells and MDA-MD-468 cells to generate cell library which are gene disrupted. Groups of cells which was disrupted a gene by CRISPR/Cas9 system were obtained. These cells were inoculated subcutaneously on the back of immunodeficient mice to grow tumors. Even NIH3T3 cells which were introduced library failed to stable tumorigenesis as well as wild type cells. These data suggested that NIH3T3 population containing different kinds of a single gene disrupted cells did not achieve capability of tumorigenesis. With MDA-MD-462 cells, we observed cells introduced CRISPR/Cas9 library gain function of tumorigenesis compared to wild-type cells.
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| Free Research Field |
細胞増殖メカニズムやそれを修飾する分子に関わる研究
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