2017 Fiscal Year Final Research Report
A unified understanding of replication error correction and antirecombination mediated by the mismatch repair system
| Project/Area Number |
16K14671
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
Oda Satomi
Kawasoe Yoshitaka
Sakazume Aya
Terui Riki
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | ミスマッチ修復 / 相同組換え / ツメガエル卵抽出液 / 無細胞系 / DNA二重鎖切断損傷 |
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| Outline of Final Research Achievements |
The mismatch repair system not only increases the replication fidelity by correcting replication errors but also improves the recombination fidelity by preventing illegitimate recombination. To study how these reactions are branched and how illegitimate recombination is inhibited, we established an in vitro model system that recapitulates the inhibition of recombination between divergent sequences. We induced single-strand annealing repair of DNA double-strand breaks by adding DNA substrates into nucleoplasmic extracts of Xenopus eggs and found that sequence divergence between the annealing units significantly reduces the annealing reaction. We also found that the mismatch-recognition complex and a RecQ-family helicase prevent annealing of divergent sequences. This system will be useful to study the recombination intermediates and factors that are involved in the inhibition of illegitimate recombination.
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| Free Research Field |
分子遺伝学
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