2017 Fiscal Year Final Research Report
Study on functions generated by suppressing diffusive motion of proteins by interaction with intracellular structures
| Project/Area Number |
16K14704
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Muroran Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | タンパク質 / 拡散運動 / 自己集合 / 細胞骨格 / 微小管 / アクチン / 細胞膜 / アミロイド |
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| Outline of Final Research Achievements |
In this study, we analyzed how proteins interact with each other and express their functions under conditions where the diffusive motion of protein molecules is spatially restricted as in vivo. Microtubule assembly-promoting activity of MAP2 and MAP4 with high affinity for F-actin was enhanced in the presence of F-actin, but tau with low affinity for F-actin was not. Besides, when aggregation of amyloid β was real-time imaged using quantum dots, it was observed that aggregation was promoted in the presence of cultured cells. These results suggested that suppression of diffusion of protein molecules by interaction with F-actin and cell membrane greatly affects expression of the protein functions.
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| Free Research Field |
生物物理学
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