Developing the method for plastid transformation of rice

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K14828
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: The University of Tokyo
• Principal Investigator: Tsutsumi Nobuhiro 東京大学, 大学院農学生命科学研究科(農学部), 教授 (00202185)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: プラスチド / 形質転換 / オルガネラ / 選抜マーカー

Outline of Final Research Achievements

For the purpose of developing rice plastid transformation method, we improved and examined cultivation methods and transgene selection systems. First, in order to improve gene transfer efficiency, a culture system of plastid developed green tissue was examined, and the shoot apex with developing leaves and a multiple shoot induction culture system of the seedling were developed. In addition, plastid transformation by the newly developed nitrite selection method using the conventional rice callus was carried out and it succeeded in highly efficient gene introduction. It was also confirmed that the transgene was retained in the next generation (T1). Through the detailed analysis of transformed plants, we will further study the improvement to establish the plastid transformation method in rice.

Free Research Field

遺伝育種科学