Molecular mechanism of survival strategy of phyllosphere methylotrophic yeasts exposed to harsh environmental changes and stresses

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14883
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: SAKAI Yasuyoshi 京都大学, 農学研究科, 教授 (60202082)
• Co-Investigator(Renkei-kenkyūsha): YURIMOTO Hiroya 京都大学, 大学院農学研究科, 准教授 (00283648) ; OKU Masahide 京都大学, 大学院農学研究科, 助教 (10511230)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ストレス顆粒 / メタノール / Candida boidinii / Pichia pastoris

Outline of Final Research Achievements

This study aimed to elucidate the molecular mechanism of survival strategy of methylotrophic yeasts which can survive and proliferate on growing plant leaves where cells are exposed to harsh environmental changes and stresses. Physiological role of stress granule (SG) formation and methanol-sensing mechanism in methylotrophic yeasts were investigated. We revealed that sequestration of activated Hog1 proteins in SGs is responsible for downregulation of Hog1 activity under high-temperature stress and that Wsc family proteins function in both the high temperature stress response and in methanol-sensing machinery.

Free Research Field

応用微生物学