Detection and identification of complement components in bony fish skin mucus and their interactome analysis

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K14985
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Kyushu University
• Principal Investigator: NAKAO Miki 九州大学, 農学研究院, 教授 (50212080)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 免疫 / 生体防御 / 補体 / 体表 / 粘液 / インタラクトーム / 相互作用 / 魚類

Outline of Final Research Achievements

The complement system is a major humoral factor in innate immunity, and in mammals its components are mainly synthesized in the liver and are thought to function in body fluids such as plasma. In fish, gene expression of complement components has been confirmed in organs and regions other than liver, but there are many unknown points about the distribution in the fish of the complement component protein and the function in the area. In this study, we focused on body surface mucus, which is the first barrier in the body defense, and aimed to identify the complement components present in mucus and clarify the reaction mechanism. As the first step, it was attempted to clarify the presence of complement components in carp surface mucus at the protein level by Western blot using antibodies against each complement component of carp.

Free Research Field

比較免疫学

Academic Significance and Societal Importance of the Research Achievements

本研究では、魚類の体表、生殖腺、脳などで発現することが判明している補体成分の、それら局所での未知の機能を明らかにするために、局所で補体成分が相互作用する分子を網羅的に探索した。このアプローチは、既知の補体系内での機能という先入観にとらわれず、補体成分の新機能に推定することができる。本研究の考え方と方法論は、補体成分のみならず、ゲノムデータ中で配列と発現部位しかわかっていない多くの機能未知遺伝子について、それらの生物学的役割りに迫る一般的な研究戦略として展開可能となる。