High-speed super-resolution microscopy to visualize living lipid microdomain

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15103
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: Otomo Kohei 北海道大学, 電子科学研究所, 助教 (40547204)
• Co-Investigator(Renkei-kenkyūsha): NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 二光子顕微鏡 / 超解像顕微鏡 / STED顕微鏡 / スピニングディスク顕微鏡 / in vivoイメージング

Outline of Final Research Achievements

This study aimed at developing novel two-photon excitation microscopy which possesses the spatial resolution overcoming diffraction limits and millisecond temporal resolution to visualize living lipid microdomain in real time. To accomplish the purpose, we further improved both two-photon excitation stimulated emission depletion (STED) microscopy and multi-point scanning two-photon excitation microscopy, which we had developed so far. Although we have not developed the initially planned microscopy system which combined both technologies, resolutions and utilities of respective microscopy systems were successfully improved. Especially, the latter multi-point scanning system could be utilized for general purposes, thus we started to open this system as the equipment of Nikon imaging center at Hokkaido University.

Free Research Field

生物物理学