• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2017 Fiscal Year Final Research Report

Analysis of the mechanism of a novel DNA repair reaction in mammalian cells

Research Project

  • PDF
Project/Area Number 16K15118
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionKanazawa University

Principal Investigator

MATSUNAGA Tsukasa  金沢大学, 薬学系, 教授 (60192340)

Co-Investigator(Kenkyū-buntansha) 若杉 光生  金沢大学, 薬学系, 准教授 (80345595)
Project Period (FY) 2016-04-01 – 2018-03-31
Keywordsヌクレオチド除去修復 / 紫外線 / DNA損傷 / 色素性乾皮症
Outline of Final Research Achievements

We recently showed that mammalian cells slowly remove UV-induced 6-4 photoproducts from their genome under the nucleotide excision repair (NER)-defective condition. In this study, we have tried to uncover the mechanism of the NER-independent reaction in human cells. We have found that the non-canonical repair reaction requires XPC, but not DDB, both of which are well-known NER factors involved in a damage recognition step. We further found that a proteasomal activity is also required for this reaction only when NER-deficient cells have functional DDB, suggesting the implication of proteasomal degradation mediated by Cul4-DDB1 E3 ligase.

Free Research Field

分子細胞生物学

URL: 

Published: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi