2017 Fiscal Year Final Research Report
A study on antifungal effect of apple fruit polyphenol
| Project/Area Number |
16K15186
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 真菌 / ポリフェノール / 果実 / 蛍光グルコース |
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| Outline of Final Research Achievements |
A piece of specimen was cultured after dissecting out from apple fruit, which had been naturally cultivated under a no pesticide-, no fertilizer-condition. Although the specimen had been disinfected from the outside when cultured just after the dissection, fungal spores appeared possibly from the inside of the specimen. Incidentally, an apple fruit polyphenol in exocarp inhibits bacterial sugar metabolism. Bacterial metabolites in turn suppress a sugar transporter of fungi. As such, I hypothesized that apple fruit could well suppress fungi indirectly through regulation of the bacteria with the polyphenol. To test this, I planned to explore the interaction of these microorganisms with fluorescent sugar derivatives. Preliminary experiments, however, revealed that the sugar uptake was too heterogeneous to see with a conventional method, making us to develop a new three-dimensional, multiple angle imaging method. We hope this method will be a tool to elucidate the complex interaction.
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| Free Research Field |
生理学
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