2016 Fiscal Year Final Research Report
Screening for gut microbiota-derived metabolites that stimulate intestinal stem cells
| Project/Area Number |
16K15422
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2017-03-31
|
| Keywords | Wntシグナル / 腸管幹細胞 / 常在性腸内細菌叢 / 生理活性代謝産物 |
|---|
| Outline of Final Research Achievements |
This study is being conducted to identify gut microbiota-derived metabolites that stimulate intestinal stem cells. Utilizing a novel assay system targeting R-spondin-LGR4/5/6-ZNRF3/RNF43 axis, we first screened a small-molecule compound library and identified 2 unique compounds that enhance R-spondin activities. This proof-of-concept experiment suggests that this newly established assay is well suited for biochemical purification of bioactive metabolites from crude microbiota-derived extracts. We also conducted comprehensive metabolomic analysis and identified 54 metabolites from fecal extracts of germ-free mice that had received fecal microbiota transplantation (FMT). The biological activities of these metabolites are currently being examined.
|
| Free Research Field |
消化器内科学、消化器内視鏡学
|
