Explore of a novel molecule regulating Langerhans cells by visualizing method

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K15495
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: NAKAO KAZUWA 京都大学, 医学研究科, 特任教授 (00172263)
• Co-Investigator(Renkei-kenkyūsha): KANDA HAJIME 京都大学, 大学院医学研究科, 客員研究員 (00724535)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: 膵ランゲルハンス島 / ゼブラフィッシュ / アルファ細胞 / ベータ細胞 / デルタ細胞 / 共焦点レーザー顕微鏡 / フローサイトメトリー / セルソーター

Outline of Final Research Achievements

We developed a triple transgenic zebrafish expressing three types of fluorescent reporter protein for alpha cell, beta cell and delta cell of Langerhans islets of pancreas. Our research purpose was elucidation of molecular mechanism regulating volume and ratio of alpha, beta and delta cell in a Langerhans islet. Imaging analysis of 5dpf, 7dpf and 9dpf triple transgenic zebrafish was performed, and we found that the number of alpha cell was significantly increased than beta cells from 5dpf to 7dpf. To analyze molecular mechanism to regulate alpha cell and beta cell ratio, we developed sorting methods to analyze isolated cells of Langerhans of islet by Flow cytometry. Our result and developed methods have a potential to contribute future researches of Langerhans islet cells.

Free Research Field

内分泌学