2017 Fiscal Year Final Research Report
Construction of a new hematopoietic stem cell analysis system with multifunctional TVA system
| Project/Area Number |
16K15501
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 造血幹細胞 / RCAS TVA システム |
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| Outline of Final Research Achievements |
CK7-TVA knock-in ES cells were prepared by inserting a sequence coding for TVA connected to the self-cleaving peptide T2A at the termination codon portion of the CK7 locus. They were found to be capable of gene transfer by RCAS virus. Subsequently, CK7-TVA knock-in ES cells were injected into mouse blastocysts to obtain chimeric mice. Recently, it has been reported that the transcription factor Evi-1 shows hematopoietic stem cell-specific expression in blood cells. Therefore, like CK7, ES cells and mice knocking in TVA at the Evi-1 locus were prepared. These results were published in Scientific reports in 2017.
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| Free Research Field |
血液内科
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